Environmental Stress Affects the Formation of Staphylococcus aureus Persisters Tolerant to Antibiotics
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F18%3A10385883" target="_blank" >RIV/00216208:11130/18:10385883 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/18:10385883 RIV/00064203:_____/18:10385883
Result on the web
<a href="https://doi.org/10.1089/mdr.2017.0064" target="_blank" >https://doi.org/10.1089/mdr.2017.0064</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1089/mdr.2017.0064" target="_blank" >10.1089/mdr.2017.0064</a>
Alternative languages
Result language
angličtina
Original language name
Environmental Stress Affects the Formation of Staphylococcus aureus Persisters Tolerant to Antibiotics
Original language description
The ability to form persisters has been observed in many microorganisms, including Staphylococcus aureus, mainly in the context of chronic infections and the pathogenicity of these microbes. In our research, we have demonstrated that salt or oxidative stress could play a role in the formation of S. aureus persisters outside the host's intracellular interface. We pre-exposed planktonic growing bacterial culture to an oxidative or salt stress and monitored the dynamics of persister formation after ciprofloxacin and gentamicin treatment. In parallel, using the quantitative PCR (qPCR) approach, we determined the expression level of the stress sigma factor SigB. The pre-exposure of bacteria to salt stress caused a 1-2.5 order of magnitude increase in persister formation in the bacterial population after antibiotic exposure, depending on the type and concentration of the antibiotic used. In contrast, oxidative stress only minimally influenced the formation of persisters, without correlation to the antibiotic type and concentration. We have demonstrated that both stress and antibiotic exposure increase the expression of sigB in bacterial populations from very early on. And that the expression level of sigB differs with the type of antibiotic and stress, but no correlation was observed between persister formation and sigB expression. The method used could be helpful in testing the ability that strains can have, to form persisters.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NV17-30460A" target="_blank" >NV17-30460A: Molecular characterization of adaptive antibiotic resistance and tolerance mechanisms of Staphylococcus aureus strains causing chronic infections</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial Drug Resistance
ISSN
1076-6294
e-ISSN
—
Volume of the periodical
24
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
547-555
UT code for WoS article
000434256200005
EID of the result in the Scopus database
2-s2.0-85048061672