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Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11130%2F20%3A10410931" target="_blank" >RIV/00216208:11130/20:10410931 - isvavai.cz</a>

  • Alternative codes found

    RIV/00064203:_____/20:10410931

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CGwXsuLjRx" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CGwXsuLjRx</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/bs.mie.2019.05.025" target="_blank" >10.1016/bs.mie.2019.05.025</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

  • Original language description

    Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMPs) to the tumor microenvironment. Once emitted by dying cancer cells, DAMPs orchestrate antigen-specific immune responses by acting on both innate and adaptive components of the immune system. Accumulating preclinical and clinical evidence indicates that one of these DAMPs, calreticulin (CALR) represents a novel powerful prognostic biomarker, reflecting the activation of a clinically relevant anticancer immune response in different cancer malignancies. Therefore, the assessment of CALR emission can provide a therapeutic tool for the stratification of cancer patients and the identification of individuals that are intrinsically capable to respond to a particular treatment. Here we describe methods for the quantification of CALR exposure in the tumor microenvironment of cancer patients by flow cytometry and immunohistochemistry. (C) 2020 Elsevier Inc.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30102 - Immunology

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods in Enzymology

  • ISSN

    0076-6879

  • e-ISSN

  • Volume of the periodical

    632

  • Issue of the periodical within the volume

    Cellular Methods Pt B

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    15-25

  • UT code for WoS article

    000556278200003

  • EID of the result in the Scopus database

    2-s2.0-85068363194