Detection of Carbapenemases Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F15%3A10279699" target="_blank" >RIV/00216208:11140/15:10279699 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/book/10.1007/978-1-4939-1776-1" target="_blank" >http://link.springer.com/book/10.1007/978-1-4939-1776-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-1776-1_9" target="_blank" >10.1007/978-1-4939-1776-1_9</a>
Alternative languages
Result language
angličtina
Original language name
Detection of Carbapenemases Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) Meropenem Hydrolysis Assay
Original language description
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identification of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by ?-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confirm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NT11032" target="_blank" >NT11032: Analysis of Mobile Genetic Elements Carried Out Metallo-Beta-Lactamase Genes in the Czech Republic</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Sepsis: Diagnostic Methods and Protocols
ISBN
978-1-4939-1776-1
Number of pages of the result
6
Pages from-to
91-96
Number of pages of the book
262
Publisher name
Springer
Place of publication
New York
UT code for WoS chapter
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