Selection of red deer spermatozoa with different cryoresistance using density gradients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F16%3A10324638" target="_blank" >RIV/00216208:11140/16:10324638 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/rda.12755" target="_blank" >http://dx.doi.org/10.1111/rda.12755</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/rda.12755" target="_blank" >10.1111/rda.12755</a>
Alternative languages
Result language
angličtina
Original language name
Selection of red deer spermatozoa with different cryoresistance using density gradients
Original language description
The objective of sperm selection media is selecting the best spermatozoa and to remove seminal plasma and diluent for using them in assisted reproductive techniques (ART). It is known that individuals show different cryoresistance in response to the same freezing procedure. Our hypothesis was that the efficacy of selection media could be dissimilar for samples with different sperm quality after thawing. Epididymal sperm samples from mature Iberian red deer were collected and frozen. Males were classified as with high post-thaw sperm quality when sperm motility (SM) GREATER-THAN OR EQUAL TO 70%, or as with low post-thaw sperm quality when SM LESS-THAN OR EQUAL TO 69%. Samples were centrifuged using the following density gradients (DG): Percoll(R), Puresperm(R) and BovipureTM, and several functional sperm parameters were assessed after sperm selecting and washing. Males classified with high sperm quality had higher post-thawing values (P > 0.05) for all parameters evaluated, except for linearity index (LIN), than those categorized as low sperm quality. After selection, some sperm characteristics improved (viability, apoptosis and mitochondrial activity) for both groups, showing the males with high sperm quality higher values in all sperm parameters except for kinematic traits and DNA fragmentation index (%DFI), regardless of DG . BovipureTM yield lower values of sperm motility, viability, apoptosis and mitochondrial activity in relation to Percoll(R) and Puresperm(R) considering both quality groups. There was an interaction between the type of DG and sperm quality group for sperm viability (P = 0.040) and apoptosis (P = 0.003). Thus, Percoll(R) selected less live and more apoptotic spermatozoa than Puresperm(R) and BovipureTM for males with low sperm quality. In conclusion, the DG are more efficient selecting spermatozoa from samples with high sperm quality, acting differently depending on initial sperm quality.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1503" target="_blank" >LO1503: BIOMEDIC</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction in Domestic Animals
ISSN
0936-6768
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
895-900
UT code for WoS article
000390030200005
EID of the result in the Scopus database
2-s2.0-84994104662