Role of N-Cadherin in Epithelial-to-Mesenchymal Transition and Chemosensitivity of Colon Carcinoma Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11140%2F22%3A10450913" target="_blank" >RIV/00216208:11140/22:10450913 - isvavai.cz</a>

Alternative codes found

RIV/62690094:18470/22:50019605 RIV/75010330:_____/22:00014083 RIV/00216208:11150/22:10450913 RIV/00216208:11160/22:10450913 RIV/00179906:_____/22:10450913

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FAwQG~LCUl" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=FAwQG~LCUl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/cancers14205146" target="_blank" >10.3390/cancers14205146</a>

Result language

angličtina

Original language name

Role of N-Cadherin in Epithelial-to-Mesenchymal Transition and Chemosensitivity of Colon Carcinoma Cells

Original language description

Simple Summary The role of N-cadherin expression in epithelial-to-mesenchymal transition (EMT) and related aggressive tumor colon cancer cell phenotype was investigated using various in vitro and in vivo models. With the help of several standard laboratory techniques, it was verified that an artificially increased N-cadherin expression has only a limited reprogramming potential towards colon cancer cells unlike the case where colon cancer cells present with a naturally elevated presence of N-cadherin. (1) Background: N-cadherin expression, epithelial-to-mesenchymal transition (EMT) and aggressive biological phenotype of tumor cells are linked although the underlying mechanisms are not entirely clear. (2) Methods: In this study, we used two different in vitro cell models with varying N-cadherin expression (stabilized lines and primocultures) and investigated their select biological features including the degree of their chemoresistance both in vitro as well as in vivo. (3) Results: We report that although enforced N-cadherin expression changes select morphological and behavioral characteristics of exposed cells, it fails to successfully reprogram cells to the aggressive, chemoresistant phenotype both in vitro as well as in vivo as verified by implantation of those cells into athymic mice. Conversely, primocultures of patient-colonic cells with naturally high levels of N-cadherin expression show fully aggressive and chemoresistant phenotype pertinent to EMT (in vitro and in vivo), with a potential to develop new mutations and in the presence of dysregulated regulatory pathways as represented by investigated miRNA profiles. (4) Conclusions: The presented results bring new facts concerning the functional axis of N-cadherin expression and related biological features of colon cancer cells and highlight colon cancer primocultures as a useful model for such studies.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

<a href="/en/project/NV19-08-00113" target="_blank" >NV19-08-00113: Feasibility study of next generation sequencing for individualized therapy of patients with solid tumors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cancers

ISSN

2072-6694

e-ISSN

2072-6694

Volume of the periodical

14

Issue of the periodical within the volume

20

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

5146

UT code for WoS article

000872607900001

EID of the result in the Scopus database

2-s2.0-85140637565