Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F04%3A00002083" target="_blank" >RIV/00216208:11150/04:00002083 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats

Original language description

We evaluated the effects of proteasome inhibitors - a peptide aldehyde MG132, and two vinylsulfone inhibitors ZL3VS and AdaAhx3L3VS - on protein metabolism in sepsis. Sepsis was induced in rats by caecal ligation and puncture. Parameters of protein metabolism were measured in incubated rat skeletal muscles. Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation. The ratesof protein synthesis and leucine oxidation were evaluated by incubating muslcesin medium containing L-/1-14C/leucine. In the septic muscles, all three inhibitors testedhad no effect on protein synthesis.MG132 decreased proteolysis by more then 50%, AdaAhx3L3VS by 20%, while the effect of ZL3VS was not significant. We conclude, that MG132 and AdaAhx3L3VS reversed at least partly the protein catabolism in sepsis, as they decreased the proteolysis and did not change the protein synthesis.

Czech name

Vliv inhibitorů proteasomu MG132, ZL3VS a AdaAhX3-L3VS na metabolismus proteinů u potkanů v sepsi

Czech description

V práci je posouzen účinek inhibitorů proteasomů-peptidového aldehydu MG132,2 peptidických vinylsulfonů ZL3VS a AdaAhx3L3VS na metabolismus proteinů u sepse. Sepse byla indukována ligací a následnou punkcí slepého střeva. Parametry metabolismu proteinů byly stanoveny na modelu inkubovaného kosterního svalu. Proteolýza byla vypočtena na základě uvolnění tyrosinu do inkubačního média. Proteosyntéza a oxidace leucinu pomocí L-/1-14C/leucinu. Inhibitory proteasomů neměly vliv na proteosyntézu. MG132 snížilproteolýzu o více než 50 %, AdaAhx3L3VS o 20 % a účinek ZL3VS byl nesignifikantní. Závěrem je konstatováno, že MG132 a AdaAHx3L3VS eliminovaly proteokatabolismus indukovaný sepsí. Mechanismem bylo snížení proteolýzy a ne stimulace proteosyntézy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FB - Endocrinology, diabetology, metabolism, nutrition

OECD FORD branch

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Project

<a href="/en/project/GA303%2F03%2F1512" target="_blank" >GA303/03/1512: The possibilities to affect the negative protein balance in severe illness - the effect of proteasome inhibitors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Experimental Pathology

ISSN

0959-9673

e-ISSN

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Volume of the periodical

85

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

363-371

UT code for WoS article

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EID of the result in the Scopus database

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