Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003533" target="_blank" >RIV/00216208:11150/05:00003533 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Direct effects of proteasome inhibitor AdaAhx3L3VS on protein and amino acid metabolism in rat skeletal muscle

Original language description

We investigated direct effects of peptide vinylsulfone inhibitor AdaAhx3L3VS on protein and amino acids metabolism in rat skeletal muscle.M.soleus and m.extensor digitorum longus were incubated in metidum containing 30 umol/l AdaAhx3L3VS or no inhibitors.Total proteolysis was determined according to the rates of tyrosine release into the medium during incubation.The rates of leucine oxidation and protein synthesis were evaluated during incubation in medium containing L-[1-14C]leucine.Amino acid concentrations in the medium were measured using HPLC.Statistical comparisons were performed by paired t-tests.AdaAhx3L3VS decreased both proteolysis (by 20%) and protein synthesis (by 10-20%)and increased leucine oxidation.The release of a number of amino acidsinto the medium was lowered.We conclude that AdaAhx3L3VS decreased overall protein turnover in the muscle tissue.

Czech name

Přímý účinek inhibitoru proteasomu AdaAhx3L3VS na metabolismus proteinů a aminokyselin

Czech description

V této práci jsme studovali účinek inhibitoru AdaAhx3L3VS na metabolismus proteinů a aminokyselin v kosterním svalu potkana.M.soleus a m.extensor digitorum longus byly inkubovány v médiu obsahujícím 30 uml/l AdaAhx3L3VS nebo bez inhibitor.Proteolýza bylahodnocena na základě množství tyrosinu uvolněného do inkubačního média.Oxidace leucinu a proteosyntéza byly hodnoceny pomocí L-[1-14C] leucinu.Koncentrace aminokyselin v médiu byly měřeny pomocí HPLC.Statistické hodnocení bylo provedeno párovým t-testem.Přidání AdaAhx3L3VS do inkubačního média snížilo proteolýzu o 20 % a proteosyntézu o 10-20 % a zvýšilo oxidaci leucinu.Do média se snížilo uvolňování řady aminokyselin.Uvedené výsledky ukazují,že pridání AdaAhx3L3VS k médiu snižuje obrat proteinů v inkubovaném kosterním svalu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GA303%2F03%2F1512" target="_blank" >GA303/03/1512: The possibilities to affect the negative protein balance in severe illness - the effect of proteasome inhibitors</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Physiological Research

ISSN

0862-8408

e-ISSN

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Volume of the periodical

54

Issue of the periodical within the volume

5

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

541-547

UT code for WoS article

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EID of the result in the Scopus database

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