Enzyme Activity and AGE Formation in a Model of AST Glycoxidation by D-fructose in Vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F05%3A00003537" target="_blank" >RIV/00216208:11150/05:00003537 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/05:00007092

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Enzyme Activity and AGE Formation in a Model of AST Glycoxidation by D-fructose in Vitro

Original language description

In the Non-enzymatic glycation as the chain reaction between reducing sugars and free amino groups of proteins has been shown to correlate with physiological ageing and severity of diabetes. In this paper, the effect of D-fructose as a reactive sugar onaspartate aminotransferase (AST) as a model protein was monitored by measurements of the enzyme activity and formation of fluorescent advanced glycation end products (AGEs). presence of fructose, total fluorescent AGEs concentration was significantly higher since 5th day of incubation compared to control values, respectively. Catalytic activity of AST clearly and quantitatively demonstrated functional changes in the enzyme molecule caused by structural modifications initiated by fructose, while the evaluation of AGE formation and especially that of pentosidine by fluorescence measurement was less reliable.

Czech name

Tvorba AGE a enzymová aktivita na modelu glykoxidace AST D-frukrosou in vitro

Czech description

Neenzymová glykace volných aminoskupin bílkovin redukujícími cukry koreluje s procesy stárnutí a rovněž se závažností diabetu. Sledovali jsme efekt D-fruktosy jako reaktivního cukru na aktivitu aspartátaminotransferasy (AST) jako modelové bílkoviny. Sledovali jsme tvorbu fluorescenčních produktů pokročilé glykace (AGE) a současně jsme sledovali katalytickou aktivitu AST. Při inkubaci s fruktosou stoupá koncentrace AGE od pátého dne. Pokles katalytické aktivity jasně a kvantitativně demonstruje strukturální a funkční změny v molekule enzymu a je velmi dobře použitelný pro sledování glykace.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/LN00B125" target="_blank" >LN00B125: Research Centre of Structure and Mechanism of Action of Potential Drugs</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Pharmaceutica

ISSN

1330-0075

e-ISSN

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Volume of the periodical

55

Issue of the periodical within the volume

1

Country of publishing house

HR - CROATIA

Number of pages

8

Pages from-to

107-114

UT code for WoS article

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EID of the result in the Scopus database

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