Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F07%3A00004289" target="_blank" >RIV/00216208:11150/07:00004289 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/07:00001802
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Expression of mRNAs related to connective tissue metabolism in rat hepatic stellate cells and myofibroblasts
Original language description
Hepatic stellate cells (HSC) and liver myofibroblasts (MFB) are two cell populations most likely responsible for the synthesis of most connective tissue components in fibrotic liver. Normal and carbon tetrachloride-cirrhotic livers from rats were used toprepare HSC. Liver was perfused with pronase and collagenase solutions, followed by centrifugation of the cell suspension on a density gradient. HSC were quiescent 2 days after plating on plastic but they became activated after another 5 days in culture. When the culture was passaged 5 times, its character changed profoundly as HSC were replaced by MFB. Microarray analysis was used to determine gene expression in quiescent HSC, activated HSC and MFB. The expression of 49 genes coding for connective tissue proteins, proteoglycans, metalloproteinases and their inhibitors, growth factors and cellular markers was determined in quiescent HSC, activated HSC and MFB.
Czech name
Exprese of mRNA, které mají vztah k metabolismu pojivové tkáně, v jaterních hvězdicových buňkách a myofibroblastech
Czech description
Jaterní hvězdicové buňky (HSC) a jaterní myofibroblasty (MFB) jsou dvě buněčné populace pravděpodobně zodpovědné za syntézu většiny vaziva ve fibrotických játrech. Z normálních jater potkanů i z jater cirhotických jsme izolovali HSC perfuzí roztoky pronasy a kolagenasy. Suspensi buněk jsme dělili centrifugací na hustotním gradientu. HSC byly klidové po 2 dnech kultivace ale dalších 5 dnů vedlo k jejich aktivaci. Pasážováním buněk se značné změnil jejich charakter, HSC byly nahrazeny myofibroblasty. Exprese 49 genů kódujících vazivové bílkoviny, proteoglykany, metalloproteasy a jejich inhibitory a cytokiny byla stanovena s použitím DNA mikroarrayí, a to v klidových HSC, v aktivovaných HSC a v myofibroblastech.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FP - Other medical fields
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA305%2F03%2F1513" target="_blank" >GA305/03/1513: Expression of connective tissue proteins in rat hepatic stellate cells</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental and Toxicological Pathology
ISSN
0940-2993
e-ISSN
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Volume of the periodical
58
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
263-273
UT code for WoS article
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EID of the result in the Scopus database
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