Gamma-Radiation-Induced Phosphorylation of p53 on Serine 15 Is Dose-Dependent in MOLT-4 Leukaemia Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F09%3A00208703" target="_blank" >RIV/00216208:11150/09:00208703 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/09:00002210

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Gamma-Radiation-Induced Phosphorylation of p53 on Serine 15 Is Dose-Dependent in MOLT-4 Leukaemia Cells

Original language description

Molecular indicators of the absorbed dose of ionizing radiation are powerful tools in biodosimetry. The studies reported here were undertaken with the motivation to find such a marker among the mo lecules involved in ataxia-telangiectasia mutated kinase-dependent signalling induced by ionizing radiation (ATM-kinase, checkpoint kinase-2, protein p53, and oncoprotein Mdm2). In our previous work on T-lymphocyte leukaemia MOLT-4 cells we described the mentioned molecules of ATM-dependent pathway and none of them showed a pronounced dosedependent response. Here we employed Western blotting and ELISA assay to investigate the response of post-translationally modified p53 (particularly phosphorylated on serine 15) after gamma-irradiation. We have found the amount of phosphorylated p53 to be homogenously increased after irradiation by the doses of 0.5 to 7.5 Gy. The dose-dependent response was pronounced especially after the doses up to 3.0 Gy. The presented data indicate that p53 phosphorylat

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/2B08028" target="_blank" >2B08028: New biological methods of the received dose determination</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica (Prague)

ISSN

0015-5500

e-ISSN

—

Volume of the periodical

55

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

—

UT code for WoS article

000265765100002

EID of the result in the Scopus database

—