Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10105719" target="_blank" >RIV/00216208:11150/11:10105719 - isvavai.cz</a>
Alternative codes found
RIV/00216275:25310/11:39892024
Result on the web
<a href="http://cccc.uochb.cas.cz/76/4/0277/" target="_blank" >http://cccc.uochb.cas.cz/76/4/0277/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1135/cccc2010038" target="_blank" >10.1135/cccc2010038</a>
Alternative languages
Result language
angličtina
Original language name
Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection
Original language description
We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the s?taneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 x 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0mmol/l hydrochloric acid was stable at 4 oC for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6-97.6% for GSH and 85.0-104.4% for GSSG. The linear range is 5.0-2000.0 ?mol/l, with a detection limit of 2.1 ?mol/l (signal-to-noise ratio = 3) for GSH and 2.0-250.0 ?mol/l, with a detection limit of 0.9 ?mol/l for GSS
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Collection of Czechoslovak Chemical Communications
ISSN
0010-0765
e-ISSN
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Volume of the periodical
76
Issue of the periodical within the volume
4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
18
Pages from-to
277-294
UT code for WoS article
000289355200005
EID of the result in the Scopus database
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