Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10130535" target="_blank" >RIV/00216208:11150/13:10130535 - isvavai.cz</a>

Result on the web

<a href="http://www.degruyter.com/view/j/jab.2013.11.issue-2/v10136-012-0033-8/v10136-012-0033-8.xml" target="_blank" >http://www.degruyter.com/view/j/jab.2013.11.issue-2/v10136-012-0033-8/v10136-012-0033-8.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/v10136-012-0033-8" target="_blank" >10.2478/v10136-012-0033-8</a>

Result language

angličtina

Original language name

Alpha-tomatine activates cell cycle checkpoints in the absence of DNA damage in human leukemic MOLT-4 cells

Original language description

Alpha-tomatine is a major glycoalkaloid found in the roots, leaves, stems and fruit of tomatoes Lycopersicon esculentum. Recently, alpha-tomatine has been recognized as a potential anticancer drug. In the present study, we identified the signaling cascades involved in the antitumor effect of alpha-tomatine on MOLT-4 leukemic cells. Alpha-tomatine inhibited the proliferation and decreased the viability of MOLT-4 cells in a dose-dependent manner. An increase in the activity of caspases 9 and 3/7 was not observed. However, an increase in the amount of p53 and its phosphorylation on serine 15, as well as an increased amount of mitochondrial protein PUMA was detected 4 and 24 h after exposure to alpha-tomatine at a concentration of 1-3 ?mol/l. Inhibition ofthe proliferation of MOLT-4 cells by alpha-tomatine is also associated with an increase in p21WAF1/CIP1 and the activation of Chk2. The comet assay did not detect significant amounts of single or double DNA strand breaks in cells treated

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FR - Pharmacology and apothecary chemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Biomedicine

ISSN

1214-021X

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

93-103

UT code for WoS article

000314809600005

EID of the result in the Scopus database

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