The cytotoxic effect of alfa-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896772" target="_blank" >RIV/00216275:25310/13:39896772 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.3892/or.2013.2778" target="_blank" >http://dx.doi.org/10.3892/or.2013.2778</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2013.2778" target="_blank" >10.3892/or.2013.2778</a>

Result language

angličtina

Original language name

The cytotoxic effect of alfa-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Original language description

In recent years, alfa-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of alfa-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stability in the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 mikroM but then a recovery of cells occurred. The recovery was not caused by the biotransformation of alfa-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of alfa-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of alfa-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), and no apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in alfa-tomatine-treated cells. These results support the conclusion that alfa-tomatine does not induce

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology Reports

ISSN

1021-335X

e-ISSN

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Volume of the periodical

30

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

10

Pages from-to

2593-2602

UT code for WoS article

000330226200007

EID of the result in the Scopus database

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