The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10173671" target="_blank" >RIV/00216208:11150/13:10173671 - isvavai.cz</a>
Result on the web
<a href="http://www.spandidos-publications.com/or/30/6/2593" target="_blank" >http://www.spandidos-publications.com/or/30/6/2593</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/or.2013.2778" target="_blank" >10.3892/or.2013.2778</a>

Result language
angličtina
Original language name
The cytotoxic effect of alpha-tomatine in MCF-7 human adenocarcinoma breast cancer cells depends on its interaction with cholesterol in incubation media and does not involve apoptosis induction
Original language description
In recent years, ?-tomatine has been studied for its anticancer activity. In the present study, we focused on the cytotoxic effect of ?-tomatine in the MCF-7 human breast adenocarcinoma cell line, its mechanism of action, biotransformation and stabilityin the culture medium. We observed an inhibition of cell proliferation and viability at concentrations of 6 and 9 ?M but then a recovery of cells occurred. The recovery was not caused by the biotransformation of ?-tomatine in MCF-7 cells, but by a substantial decrease in the concentration of ?-tomatine in the culture medium due to its binding with cholesterol. Regarding the mechanism of action of ?-tomatine, we observed no DNA damage, no changes in the levels of the proteins p53 and p21(WAF1/Cip1), andno apoptosis (neither activated caspase-8 and -9, nor sub-G1 peak, or morphological signs). We found a loss of ATP in ?-tomatine-treated cells. These results support the conclusion that ?-tomatine does not induce apoptosis in the MCF-7 ce
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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