Decellularized Skeletal Muscle: A Promising Biologic Scaffold for Tissue Engineering
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F17%3A10368705" target="_blank" >RIV/00216208:11150/17:10368705 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1166/jbt.2017.1588" target="_blank" >http://dx.doi.org/10.1166/jbt.2017.1588</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1166/jbt.2017.1588" target="_blank" >10.1166/jbt.2017.1588</a>
Alternative languages
Result language
angličtina
Original language name
Decellularized Skeletal Muscle: A Promising Biologic Scaffold for Tissue Engineering
Original language description
Stroma of mammalian organs including skeletal muscle is so complex in its macroscopic structure, microarchitecture and molecular composition that its detailed artificial reconstruction is out of reach. Nevertheless a removal of cells from the organ can be used to yield the extracellular matrix (ECM) mimicking many features of the original tissue complexity. We decellularized the skeletal muscle using combination of the osmotic shock, chemical and DNase treatment and obtained the bioscaffolds free of cellular and cytoplasmic components. Moreover, the scaffold ECM reflected light microscopic organization of the muscle coverings, blood vessels and peripheral nerves and also retained 3D organization of basal laminas and collagen fibrils as confirmed by transmission electron microscopy. Laminin and collagen type IV remained organized within basal laminas supporting cell attachment and navigation. Recellularization of the scaffolds with C2C12 cells in vitro confirmed cytocompatibility of the decellularized ECM as well as its ability to support basic physiological cellular functions as the myoblasts were aligned with ECM microstructure. After 12 days in vitro the samples did not show any signs of abnormalities, regression or cell death. These data strongly indicated that skeletal muscle decellularization could be considered as a promising approach in construction of bioscaffolds suitable for muscle tissue replacement.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
<a href="/en/project/GA15-09161S" target="_blank" >GA15-09161S: Muscle remodelling on the basis of extracellular matrix seeded with functionally characterized cells.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal Of Biomaterials And Tissue Engineering
ISSN
2157-9083
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
491-498
UT code for WoS article
000398930300008
EID of the result in the Scopus database
2-s2.0-85015615042