Inositol hexaphosphate limits the migration and the invasiveness of colorectal carcinoma cells in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F18%3A10383421" target="_blank" >RIV/00216208:11150/18:10383421 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/18:10383421

Result on the web

<a href="http://www.spandidos-publications.com/10.3892/ijo.2018.4488" target="_blank" >http://www.spandidos-publications.com/10.3892/ijo.2018.4488</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/ijo.2018.4488" target="_blank" >10.3892/ijo.2018.4488</a>

Result language

angličtina

Original language name

Inositol hexaphosphate limits the migration and the invasiveness of colorectal carcinoma cells in vitro

Original language description

Inositol hexaphosphate (IP6), also known as phytic acid, has been shown to exhibit anticancer effects in a number of preclinical tumor models. IP6 decreases proliferation by arresting cells in the GO/G1 phase, inhibits iron-mediated oxidative reactions, enhances differentiation and stimulates apoptosis. The present study attempted to characterize the effect of IP6 on the migration and adhesion of colon cancer SW620 cells. IP6 was assessed at concentrations of 0.2 and 1 mM during 12, 24 and 48 h of exposure. Migration ability was measured with the real-time xCELLigence Real-Time Cell Analyzer Dual Purpose system. The expression of mRNA and proteins involved in migration and cancer progression [epithelial cell adhesion molecule, intercellular adhesion molecule-1, beta-catenin, N-cadherin, E-cadherin, matrix metalloproteinase MMP-2 and MMP-9] was determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis. The changes in the expression and subcellular localization of E-cadherin were determined by indirect immunofluorescence. IP6 induced a decrease in the migration ability of the tested SW620 cell line. IP6-treated cells also showed decreased expression of N-cadherin, increased levels of E-cadherin and decreased expression of MMP-2 and MMP-9. These results indicated that IP6 has potential to modulate the migration ability and expression of markers associated with invasion in SW620 cells; however, further analysis is necessary to obtain a detailed understanding of the mechanism of action.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Oncology

ISSN

1019-6439

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

4

Country of publishing house

GR - GREECE

Number of pages

8

Pages from-to

1625-1632

UT code for WoS article

000442971100017

EID of the result in the Scopus database

2-s2.0-85052284885