A UHPLC-DAD method for quantification of berberine and protoberberine alkaloids in herbal food supplements based on Berberis aristata extract and evaluation of their biological activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F25%3A10496977" target="_blank" >RIV/00216208:11150/25:10496977 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/25:10496977
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_FwoNKi~t6" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_FwoNKi~t6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2024.107150" target="_blank" >10.1016/j.jfca.2024.107150</a>
Alternative languages
Result language
angličtina
Original language name
A UHPLC-DAD method for quantification of berberine and protoberberine alkaloids in herbal food supplements based on Berberis aristata extract and evaluation of their biological activity
Original language description
A rapid ultra-high performance liquid chromatography with diode array detection (UHPLC-DAD) method for determination of bioactive compounds in Berberis aristata extracts containing berberine, aromoline, magnoflorine, berbamine, tetrahydropalmatine, tetrahydroberberine, jatrorrhizine, palmatine, and oxyberberine was developed. The stationary phase was Ascentis Express AQ C-18 (150 x 4.6 mm, 2.7 mu m core-shell particles with embedded polar modification). The method run time with gradient elution comprising 0.1 % aqueous phosphoric acid and acetonitrile (1.0 mL/min) was 9.2 min. Ultrasound-assisted solid-liquid extraction with methanol was used for extraction. The validated method yielded recovery from 94.60 % to 104.10 % and limits of quantification from 0.125 to 0.250 mg/L. The results of berberine content ranged from 82.99 % to 107.47 % of the declared amount. An alarmingly low content 0.46 % of declared amount of extract was also found. The biological activity and cytotoxic properties of Berberis aristata extract and berberine powder on the Hep-2 cell lines was also evaluated and corelated with the content of berberine and protoberberine alkaloids in individual extracts. The majority of food extracts showed the negligible cytotoxicity for Hep-2 cell lines while one containing the highest level of jathrorrhizine showed significant cytotoxicity. However, the inhibitory effect on the Hep-2 cell lines has not been clearly demonstrated.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
1096-0481
Volume of the periodical
139
Issue of the periodical within the volume
March
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
107150
UT code for WoS article
001401666700001
EID of the result in the Scopus database
2-s2.0-85213287787