A UHPLC-DAD method for quantification of curcuminoids and piperine in food supplements based on Curcuma longa extract and evaluation of their biological activity on the Hep-2 cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F25%3A10499750" target="_blank" >RIV/00216208:11150/25:10499750 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/25:10499750
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KRHaKTewL5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=KRHaKTewL5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jfca.2025.107833" target="_blank" >10.1016/j.jfca.2025.107833</a>
Alternative languages
Result language
angličtina
Original language name
A UHPLC-DAD method for quantification of curcuminoids and piperine in food supplements based on Curcuma longa extract and evaluation of their biological activity on the Hep-2 cell lines
Original language description
This study deals with the determination of bioactive compounds in Curcuma longa extract with addition of piperine to enhance the bioavailability of curcuminoids. The supplements containing bioactive phytochemicals including curcumin, demethoxycurcumin, bisdemethoxycurcumin, tetrahydrocurcumin, and piperine were quantified by ultra-high performance liquid chromatography method with diode array detection using an Ascentis Express RP-Amide column with 2.7 mu m core-shell particles. The chromatographic analysis was performed at 30 degrees C with a run time of 12.5 min. Ultrasound-assisted solid-liquid extraction was applied to herbal extracts prior to analysis. The validated method yielded recoveries ranging from 91.52 +/- 0.54 - 97.23 +/- 0.54 %, and the limit of detection from 0.02 to 0.06 mu g/mL. The content of curcuminoids and piperine in food supplements was alarmingly low, ranging from 1.04 % to 81.22 % of the declared content. The biological activity and cytotoxic properties of Curcuma longa extracts on the Hep-2 cell lines from a laryngeal carcinoma using Neutral Red assay and WST-1 proliferation test were also evaluated and positively correlated with the content of curcuminoids in individual preparations after 48 h. The supplement containing the lowest level of curcuminoids showed negligible cytotoxicity for Hep-2 cell lines while those containing the highest level of curcuminoids showed significant cytotoxicity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/EH23_020%2F0008535" target="_blank" >EH23_020/0008535: Advanced Techniques for Biomedical Diagnostics (ATEBIO)</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Food Composition and Analysis
ISSN
0889-1575
e-ISSN
1096-0481
Volume of the periodical
145
Issue of the periodical within the volume
September
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
107833
UT code for WoS article
001501637800003
EID of the result in the Scopus database
2-s2.0-105006648121