A comparison of in vitro methods for determining the membrane receptor expression in cell lines
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F12%3A10124799" target="_blank" >RIV/00216208:11160/12:10124799 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0969805112000492" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0969805112000492</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.nucmedbio.2012.02.007" target="_blank" >10.1016/j.nucmedbio.2012.02.007</a>
Alternative languages
Result language
angličtina
Original language name
A comparison of in vitro methods for determining the membrane receptor expression in cell lines
Original language description
Introduction: Determining the number of expressed receptors per cell (NRPC) in cell lines is an important prerequisite for many experimental procedures in biomedical research. This paper focuses on the comparison of a newly developed method of determining NRPC - the Kinetic extrapolation method (KEX) - with the standard saturation method. These two methods, both based on radiolabeled ligand-receptor binding, were compared with the data on receptor expression found using quantified western blotting. Methods: Four cell lines with different expressions of epidermal growth factor receptor (EGFR) were chosen for the experiment: A431, HaCaT, HCT116 and HepG2. Two radiolabeled monoclonal antibodies specific for EGFR were used as ligands: [I-131]-cetuximab and[I-131]-panitumumab. The classic manual technique based on the saturation of cell receptors was performed on cells seeded in 24-well plates. kThe KEX method uses the LigandTracer, a special instrument which detects ligand retention in re
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP304%2F10%2F1738" target="_blank" >GAP304/10/1738: Radiolabelled neuropeptides for cancer diagnosis and treatment</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nuclear Medicine and Biology
ISSN
0969-8051
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
7
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
4
Pages from-to
893-896
UT code for WoS article
000309033800001
EID of the result in the Scopus database
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