In vitro evaluation of concentration, labeling effectiveness and stability for I-131-labeled radioimmunoassay ligand using real-time detection technology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10335293" target="_blank" >RIV/00216208:11160/17:10335293 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/17:10335293

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jlcr.3478" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jlcr.3478</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jlcr.3478" target="_blank" >10.1002/jlcr.3478</a>

Result language

angličtina

Original language name

In vitro evaluation of concentration, labeling effectiveness and stability for I-131-labeled radioimmunoassay ligand using real-time detection technology

Original language description

Radioimmunoassay belongs to the analytical method enabling highly specific and sensitive quantification of molecules. The verification of the real-time radioimmunoassay technology usefulness for ligand-quality characteristics evaluation such as concentration, influence of radiolabeling on binding affinity and stability was estimated. The anti-epidermal growth factor receptor antibody I-131-cetuximab was employed as the ligand antibody. The concentration of I-131-cetuximab was derived from the shape of binding curves coming from the ligand-receptor interaction. The binding curves also allowed the estimation of I-131-cetuximab binding affinity for different radiolabeling procedures (incubation times 1, 5, and 10minutes) in stability testing up to 96hours at 4 degrees C. The stability testing also included comparative analysis by size exclusion high-performance liquid chromatography. The assessment of cetuximab concentrations using real-time method showed acceptable accordance between real and calculated values. The real-time method revealed that 1-minute radiolabeling proved to be the optimal incubation time for direct radioiodination of cetuximab. Stability testing showed the significant change in radioligand affinity by one order at the longest incubation times (72 and 96hours). Characterization of stability and binding behavior of radiolabeled monoclonal antibodies by the verified real-time method before use in other assays may be employed to eliminate variability and suboptimal antibody performance.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Labelled Compounds and Radiopharmaceuticals

ISSN

0362-4803

e-ISSN

—

Volume of the periodical

60

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

80-86

UT code for WoS article

000393835900010

EID of the result in the Scopus database

2-s2.0-85006922964