A New and Fast HPLC Method for Determination of Rutin, Troxerutin, Diosmin and Hesperidin in Food Supplements Using Fused-Core Column Technology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F13%3A10146034" target="_blank" >RIV/00216208:11160/13:10146034 - isvavai.cz</a>

Result on the web

<a href="http://link.springer.com/article/10.1007/s12161-012-9551-y/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s12161-012-9551-y/fulltext.html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12161-012-9551-y" target="_blank" >10.1007/s12161-012-9551-y</a>

Result language

angličtina

Original language name

A New and Fast HPLC Method for Determination of Rutin, Troxerutin, Diosmin and Hesperidin in Food Supplements Using Fused-Core Column Technology

Original language description

A new and fast high-performance liquid chromatography (HPLC) method using fused-core column for separation of rutin, troxerutin, diosmin, and hesperidin has been developed and used for determination of these flavonoids in food supplements. Efficient separation of flavonoids and internal standard methylparaben was achieved on the fused-core column Ascentis Express RP-Amide (100 x 3.0 mm), particle size 2.7 mu m, with mobile phase acetonitrile/water solution of acetic acid pH 3 (30:70, v/v) at a flow rateof 1.0 mL min(-1) and at temperature 50 A degrees C. The detection wavelengths were set at 283 nm for hesperidin and at 255 nm for rutin, troxerutin, diosmin, and internal standard methylparaben. Under the optimal chromatographic conditions, good linearity with correlation coefficients in the range (r = 0.9991-0.9998; n = 7) for all flavonoids was achieved. Commercial samples of food supplements were extracted with 100 % dimethyl sulfoxide using ultrasound bath for 10 min and then dilut

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Food Analytical Methods

ISSN

1936-9751

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

1353-1360

UT code for WoS article

000324271200014

EID of the result in the Scopus database

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