A Fast Determination of Chlorophylls in Barley Grass Juice Powder Using HPLC Fused-Core Column Technology and HPTLC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F14%3A10281872" target="_blank" >RIV/00216208:11160/14:10281872 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s12161-013-9665-x/fulltext.html" target="_blank" >http://link.springer.com/article/10.1007/s12161-013-9665-x/fulltext.html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s12161-013-9665-x" target="_blank" >10.1007/s12161-013-9665-x</a>
Alternative languages
Result language
angličtina
Original language name
A Fast Determination of Chlorophylls in Barley Grass Juice Powder Using HPLC Fused-Core Column Technology and HPTLC
Original language description
This work presents a novel approach to qualitative and quantitative evaluation of chlorophyll content in food supplement using simple and fast separation techniques (high performance liquid chromatography (HPLC) and high performance thin layer chromatography (HPTLC)). For the first time, an AscentisA (R) Express C-18 HPLC column (100 x 4.6 mm; 2.7 mu m), based on fused-core particles, was utilised for the analysis of chlorophyll a and chlorophyll b in green young barley grass juice powder extracts. Themobile phase was composed of methanol, water and ethyl acetate of 40:10:50 (v/v/v) at a flow rate of 1.5 mL/min. Chlorophylls were separated in time less than 6 min. The extraction procedure was optimised by using of 90 % of acetone in water, and the method was validated. The method precision was found in the range of 1.15-2.85 %, and the method recovery was established at three concentration levels. Calibration graphs were linear in the range of 0.75-25.5 mg/L, with correlation coeffici
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Food Analytical Methods
ISSN
1936-9751
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
629-635
UT code for WoS article
000330734300012
EID of the result in the Scopus database
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