Evaluation of 2,6-dichlorophenolindophenol acetate as a substrate for acetylcholinesterase activity assay

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F15%3A10306834" target="_blank" >RIV/00216208:11160/15:10306834 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/15:43875420

Result on the web

<a href="http://www.tandfonline.com/doi/full/10.3109/14756366.2014.976564" target="_blank" >http://www.tandfonline.com/doi/full/10.3109/14756366.2014.976564</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3109/14756366.2014.976564" target="_blank" >10.3109/14756366.2014.976564</a>

Result language

angličtina

Original language name

Evaluation of 2,6-dichlorophenolindophenol acetate as a substrate for acetylcholinesterase activity assay

Original language description

Ellman's method is a standard protocol for the determination of cholinesterases activity. Though the method is ready for laboratory purposes, it has some drawbacks as well. In the current article, 2,6-dichloroindophenol acetate is performed as a chromogenic substrate suitable for acetylcholinesterase (AChE) activity examination. Michaelis constant and maximal velocity for 2,6-dichloroindophenol acetate were determined (38.0 mu M and 244 pkat) and compared to the values for acetythiocholine (Km 0.18 mM; Vmax 5.1 nkat). Docking for 2,6-dichloroindophenol acetate and human AChE was done as well. In conclusion, 2,6-dichloroindophenol acetate seems to be suitable chromogenic substrate for AChE and spectrophotometry and based on this it can be easily performed whenever AChE activity should be tested.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/EE2.3.20.0235" target="_blank" >EE2.3.20.0235: Establishment of Research Team Focused on Experimental and Applied Biopharmacy</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Enzyme Inhibition and Medicinal Chemistry

ISSN

1475-6366

e-ISSN

—

Volume of the periodical

30

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

4

Pages from-to

796-799

UT code for WoS article

000361328700011

EID of the result in the Scopus database

2-s2.0-84940662635