A Smartphone Camera Colorimetric Assay of Acetylcholinesterase and Butyrylcholinesterase Activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F21%3A00557416" target="_blank" >RIV/60162694:G44__/21:00557416 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1424-8220/21/5/1796" target="_blank" >https://www.mdpi.com/1424-8220/21/5/1796</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/s21051796" target="_blank" >10.3390/s21051796</a>
Alternative languages
Result language
angličtina
Original language name
A Smartphone Camera Colorimetric Assay of Acetylcholinesterase and Butyrylcholinesterase Activity
Original language description
Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) can serve as biochemical markers of various pathologies like liver disfunction and poisonings by nerve agents. Ellman's assay is the standard spectrophotometric method to measure cholinesterase activity in clinical laboratories. The authors present a new colorimetric test to assess AChE and BChE activity in biological samples using chromogenic reagents, treated 3D-printed measuring pads and a smartphone camera as a signal detector. Multiwell pads treated with reagent substrates 2,6-dichlorophenolindophenyl acetate, indoxylacetate, ethoxyresorufin and methoxyresorufin were prepared and tested for AChE and BChE. In the experiments, 3D-printed pads containing indoxylacetate as a chromogenic substrate were optimal for analytical purposes. The best results were achieved using the red (R) channel, where the limit of detection was 4.05 mu kat/mL for BChE and 4.38 mu kat/mL for AChE using a 40 mu L sample and a 60 min assay. The major advantage of this method is its overall simplicity, as samples are applied directly without any specific treatment or added reagents. The assay was also validated to the standard Ellman's assay using human plasma samples. In conclusion, this smartphone camera-based colorimetric assay appears to have practical applicability and to be a suitable method for point-of-care testing because it does not require specific manipulation, additional education of staff or use of sophisticated analytical instruments.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/TH03030336" target="_blank" >TH03030336: Colorimetric sensor for diagnosis of pesticides poisoning</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Sensors
ISSN
1424-8220
e-ISSN
1424-8220
Volume of the periodical
21
Issue of the periodical within the volume
5
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
1796
UT code for WoS article
000628538600001
EID of the result in the Scopus database
2-s2.0-85101956430