Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F15%3A43875381" target="_blank" >RIV/60162694:G44__/15:43875381 - isvavai.cz</a>

Result on the web

<a href="http://www.degruyter.com/view/j/chempap.2015.69.issue-8/chempap-2015-0117/chempap-2015-0117.xml" target="_blank" >http://www.degruyter.com/view/j/chempap.2015.69.issue-8/chempap-2015-0117/chempap-2015-0117.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/chempap-2015-0117" target="_blank" >10.1515/chempap-2015-0117</a>

Result language

angličtina

Original language name

Determination of acetylcholinesterase and butyrylcholinesterase activity without dilution of biological samples

Original language description

Two cholinesterases: acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), are known. The enzymes are important in the body and alteration of their activity has significant use in the diagnosis of poisoning, liver function, etc. Currently available methods for the determination of cholinesterases have some major drawbacks including various interferences and the inability to be used for decreasing the enzyme activity in the presence of reversible inhibitors due to sample dilution; hence, a method for dilution free assay of cholinesterases is desired. Here, microplates were modified with indoxylacetate (100 mu L of 10 mmol L-1 solution) and used for cholinesterases assay after drying at 37 degrees C. The fact that indoxylacetate remains stable in dry state and serves simultaneously as a chromogen and substrate provide good prerequisites for the method. The limit of detection for BChE was 0.71 U while that for AChE was 2.8 U per a 100 mu L sample (solution of enzyme or plasma sample). The limit of detection is low enough to allow standard examination of cholinesterasemia. The two cholinesterases can be distinguished from each other using selective inhibitors such as donepezil and iso-OMPA. The new method was also successfully validated for the standard Ellman's assay using plasma samples with BChE activity adjusted by carbofuran. The new method based on indoxylacetate seems promising for routine tests. (C) 2015 Institute of Chemistry, Slovak Academy of Sciences

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical Papers-Chemicke Zvesti

ISSN

0366-6352

e-ISSN

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Volume of the periodical

69

Issue of the periodical within the volume

8

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

1044-1049

UT code for WoS article

000354752200004

EID of the result in the Scopus database

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