A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F23%3A00558022" target="_blank" >RIV/60162694:G44__/23:00558022 - isvavai.cz</a>
Result on the web
<a href="https://www.hindawi.com/journals/ijac/2022/2623155/" target="_blank" >https://www.hindawi.com/journals/ijac/2022/2623155/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1155/2022/2623155" target="_blank" >10.1155/2022/2623155</a>
Alternative languages
Result language
angličtina
Original language name
A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay
Original language description
Biosensors containing cholinesterase are analytical devices suitable for the assay of neurotoxic compounds. In the research on biosensors, a new platform has appeared some years ago. It is the digital photography and scoring of coloration (photogrammetry). In this paper, a colorimetric biosensor is constructed using 3D-printed multiwell pads treated with indoxylacetate as a chromogenic substrate and gold nanoparticles with the immobilized enzyme butyrylcholinesterase. A smartphone camera served for photogrammetry. The biosensor was tested for the assay of carbofuran and paraoxon ethyl as two types of covalently binding inhibitors: irreversible and pseudoirreversible. The biosensor exerted good sensitivity to the inhibitors and was able to detect carbofuran with a limit of detection for carbofuran 7.7 nmol/l and 17.6 nmol/l for paraoxon ethyl. A sample sized 25 μl was suitable for the assay lasting approximately 70 minutes. Up to 121 samples can be measured contemporary using one multiwell pad. The received data fully correlated with the standard spectrophotometry. The colorimetric biosensor exerts promising specifications and appears to be competitive to the other analytical procedures working on the principle of cholinesterase inhibition. Low-cost, simple, and portable design represent an advantage of the assay of the biosensor. Despite the overall simplicity, the biosensor can fully replace the standard spectroscopic methods.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Analytical Chemistry
ISSN
1687-8760
e-ISSN
1687-8779
Volume of the periodical
2022
Issue of the periodical within the volume
Apr
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
2623155
UT code for WoS article
000791186300002
EID of the result in the Scopus database
2-s2.0-85128595004