Digital camera-based lipase biosensor for the determination of paraoxon

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F18%3A43889567" target="_blank" >RIV/60162694:G44__/18:43889567 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/18:00103400

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S0925400518311894?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0925400518311894?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2018.06.084" target="_blank" >10.1016/j.snb.2018.06.084</a>

Result language

angličtina

Original language name

Digital camera-based lipase biosensor for the determination of paraoxon

Original language description

This work is focused on construction of a biosensor containing unique bacterial homogenate with high lipase activity and colorimetric type of assay where camera of a smartphone was chosen as a detector. The biosensor was constructed as a tool of lipase inhibitors and paraoxon served as a representative analyte inhibiting the lipase. Psychrophilic strains of bacteria isolated from in Antarctica were tested and the best isolate P4368 having huge lipase activity was chosen. Tween assay was performed as a standard method for lipase activity determination and indoxylacetate served as a substrate of lipase measurable by smartphone camera and R, G and B color channels digital analysis. Bacteria were homogenized and immobilized on polyvinylidene difluoride membrane and indoxylacetate was immobilized in the proximity of the homogenate. Paraoxon ethyl was analyzed by the standard method and by the camera-based biosensor. The biosensor-based assay was found to determine paraoxon with limit of detection 3.72 x 10(-8) mol/l and IC50 value for paraoxon was 4.00 x 10(-6) mol/l. A volume 5 mu l of sample was sufficient for the assay and the sample was applied directly without any processing. In a conclusion, a simple colorimetric biosensor for the determination of venomous compounds like organophosphorus pesticides and nerve agents was constructed and promising analytical parameters were received during its characterization.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LM2015078" target="_blank" >LM2015078: Czech Polar Research Infrastructure</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B-Chemical

ISSN

0925-4005

e-ISSN

—

Volume of the periodical

273

Issue of the periodical within the volume

Nov

Country of publishing house

CH - SWITZERLAND

Number of pages

6

Pages from-to

610-615

UT code for WoS article

000441519000076

EID of the result in the Scopus database

2-s2.0-85048991004