Assessment of acetylcholinesterase activity using Indoxylacetate and comparison with the standard Ellman's method

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F11%3A00002493" target="_blank" >RIV/60162694:G44__/11:00002493 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3390/ijms12042631" target="_blank" >http://dx.doi.org/10.3390/ijms12042631</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Assessment of acetylcholinesterase activity using Indoxylacetate and comparison with the standard Ellman's method
Original language description
Assay of acetylcholinesterase (AChE) activity plays an important role in diagnostic, detection of pesticides and nerve agents, in vitro characterization of toxins and drugs including potential treatments for Alzheimer's disease. These experiments were done in order to determine whether indoxylacetate could be an adequate chromogenic reactant for AChE assay evaluation. Moreover, the results were compared to the standard Ellman's method. We calculated Michaelis constant Km (2.06 x 10-4 mol/L for acetylthiocholine and 3.21 x 10-3 mol/L for indoxylacetate) maximum reaction velocity Vmax (4.97 x 10-7 kat for acetylcholine and 7.71 x 10-8 kat for indoxylacetate) for electric eel AChE. In a second part, inhibition values were plotted for paraoxon, and reactivation efficacy was measured for some standard oxime reactivators: obidoxime, pralidoxime (2-PAM) and HI-6. Though indoxylacetate is split with lower turnover rate, this compound appears as a very attractive reactant since it does not show
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/OVUOFVZ200807" target="_blank" >OVUOFVZ200807: Biosensors for determination of nerve agents and yperites using recombinant proteins and nanotechnology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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