Simple and rapid quantification of vancomycin in serum, urine and peritoneal/pleural effusion via UHPLC-MS/MS applicable to personalized antibiotic dosing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F17%3A10362437" target="_blank" >RIV/00216208:11160/17:10362437 - isvavai.cz</a>
Alternative codes found
RIV/00179906:_____/17:10362437 RIV/00216208:11150/17:10362437
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0731708517306568" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0731708517306568</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2017.04.029" target="_blank" >10.1016/j.jpba.2017.04.029</a>
Alternative languages
Result language
angličtina
Original language name
Simple and rapid quantification of vancomycin in serum, urine and peritoneal/pleural effusion via UHPLC-MS/MS applicable to personalized antibiotic dosing
Original language description
Management of the therapy of life-threatening bacterial infection is extremely based on an optimal antibiotic treatment. Achieving the correct vancomycin dosage in blood and target tissues can be complicated in special situations, e.g., where large fluid sequestration and/or acute renal failure occur. A UHPLC-MS/MS method operating in electrospray (ESI) positive ion mode was applied for the determination of vancomycin in serum, urine and peritoneal/pleural effusion. Sample pretreatment was composed of dilution and simple protein precipitation where only a small volume (50 mu L) of serum, urine or peritoneal/pleural effusion was required. The separation of vancomycin was performed on a Meteoric Core C18 BIO column (100 x 4.6 mm, 2.7 mu m) by gradient elution with 0.1% formic acid in water and acetonitrile. The total time of analysis was 4.5 min. The method was found to be linear in the range of 2-60 mu M (or 0.5-10 mu M) for serum, 0.27-10 mu M (or 2-60 mu M) for peritoneal/pleural effusion and 25-300 mu M for urine, which was adequate for the determination of vancomycin in patient samples. The intra- and inter-day precision was below 8% RSD, and accuracy was from 89 to 104%. The UHPLC/MS-MS method offers a fast and reliable approach to determine vancomycin concentrations in three different human body fluid samples (serum, urine and peritoneal/pleural effusion) with a simple sample pretreatment that was the same for all selected specimens. This method should be applicable to large sample series in clinical (pharmacokineticipharmacodynamic) studies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/NT14089" target="_blank" >NT14089: Personalized antibiotic therapy in surgical patients with heavy bacterial infection and important sequestration of liquids.</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
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Volume of the periodical
142
Issue of the periodical within the volume
August
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
59-65
UT code for WoS article
000403384400009
EID of the result in the Scopus database
2-s2.0-85018381315