Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10410695" target="_blank" >RIV/00216208:11310/20:10410695 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/20:10410695 RIV/00023728:_____/20:N0000058
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fFS_Aw8XCr" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=fFS_Aw8XCr</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2019.113509" target="_blank" >10.1016/j.ab.2019.113509</a>
Alternative languages
Result language
angličtina
Original language name
Rapid and reliable HILIC-MS/MS method for monitoring allantoin as a biomarker of oxidative stress
Original language description
Allantoin is an excellent biomarker of oxidative stress in humans as the main product of uric acid oxidation by reactive oxygen species. Yet, allantoin determination is still not routinely performed in clinical laboratories. Therefore, we developed a fast, simple, selective, and sensitive UHPLC-MS/MS method for allantoin determination in human serum using an isotopically labeled internal standard. Our analytical protocol provided high sensitivity by mass spectrometry detection and high throughput by HILIC-MS/MS analysis within 4 min, with one-step serum sample preparation approximately within 7 min. Lastly, our protocol was fully validated to demonstrate its reliability in allantoin determination in human serum. The method showed an excellent linear range from 0.05 to 100 mu M, with precision ranging from 1.8 to 11.3% (RSD), and with accuracy (relative error %) within +/- 6.0%. The method was then applied to analyze the concentration of allantoin in serum samples from 71 patients with chronic gout without treatment with xanthine oxidase inhibitors. The median serum allantoin concentration in the cohort was 2.8 mu M (n = 71). Overall, our simple analytical protocol has the potential to be easily implemented in clinical routine practice for monitoring allantoin as a key oxidative stress biomarker.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GJ19-18005Y" target="_blank" >GJ19-18005Y: Retention mechanisms of intact glycopeptides in hydrophilic interaction liquid chromatography</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical Biochemistry
ISSN
0003-2697
e-ISSN
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Volume of the periodical
589
Issue of the periodical within the volume
January
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
113509
UT code for WoS article
000518179100012
EID of the result in the Scopus database
2-s2.0-85075214563