Hematoxylin assay of cupric chelation can give false positive results
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F19%3A10399578" target="_blank" >RIV/00216208:11160/19:10399578 - isvavai.cz</a>
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CUMhbu5b9n" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=CUMhbu5b9n</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jtemb.2018.10.022" target="_blank" >10.1016/j.jtemb.2018.10.022</a>
Alternative languages
Result language
angličtina
Original language name
Hematoxylin assay of cupric chelation can give false positive results
Original language description
Some compounds without apparent chelation sites have been shown to chelate cupric ions using the hematoxylin assay. Four different known reducing agents (hydroxylamine, vitamin C, trolox, reduced glutathione /GSH/) were selected for the study together with oxidized glutathione (GSSG). All tested compounds behaved as cupric chelators in the spectrophotometric mildly competitive hematoxylin assay. In-depth analysis however showed that only GSH and GSSG were able to form complexes with both cupric and cuprous ions and only GSSG partly retained copper in its complexes in the more competitive bathocuproine assay. All tested compounds with the exception of GSSG reduced Cu2+ ions. Compounds reducing copper such as antioxidants can give false positive results in the hematoxylin-screening assay.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
<a href="/en/project/GA18-00121S" target="_blank" >GA18-00121S: The role of optically pure flavonolignans in cardiovascular effects of silymarin</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Trace Elements in Medicine and Biology
ISSN
0946-672X
e-ISSN
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Volume of the periodical
52
Issue of the periodical within the volume
March
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
29-36
UT code for WoS article
000463958300006
EID of the result in the Scopus database
2-s2.0-85056737918