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Novel application of capillary electrophoresis with a liposome coated capillary for prediction of blood-brain barrier permeability

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11160%2F20%3A10417669" target="_blank" >RIV/00216208:11160/20:10417669 - isvavai.cz</a>

  • Alternative codes found

    RIV/00179906:_____/20:10417669

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y4OW8IJ7R4" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y4OW8IJ7R4</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.talanta.2020.121023" target="_blank" >10.1016/j.talanta.2020.121023</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Novel application of capillary electrophoresis with a liposome coated capillary for prediction of blood-brain barrier permeability

  • Original language description

    Profiling blood-brain barrier permeability of bioactive molecule is an important issue in early drug development, being a part of the optimization process of a compound&apos;s physicochemical properties, and hence pharmacokinetic profile. The study aimed to develop and optimize a new in vitro method for assessment of the compound&apos;s brain penetration. The tool is proposed as an alternative to the PAMPA-BBB (Parallel Artificial Membrane Permeability Assay for Blood-Brain Barrier) and based on a capillary electrochromatography (CEC) technique. It utilizes liposomes as structural substitutes of biological membranes, which are used as a capillary inner wall coating material. Following optimization of analysis conditions, migration times for a set of 25 reference drugs (mainly non-ionized in pH 7.4) were examined in a liposome coated capillary. On that basis, the retention factor (log k) was determined for each reference drug. Obtained log k values and experimentally received reference permeability parameters: log BB (in vivo data) and log P-e (PAMPA-BBB data) were compared with one another. Correlation coefficients were calculated, giving comparable results for CEC log k/log BB and analogical PAMPA-BBB log P-e/log BB analyses. Approximate ranges of log k for the central nervous system (CNS) permeable (CNS (+)) and non-permeable (CNS(-)) drugs were established.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30104 - Pharmacology and pharmacy

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Talanta

  • ISSN

    0039-9140

  • e-ISSN

  • Volume of the periodical

    217

  • Issue of the periodical within the volume

    SEP

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    14

  • Pages from-to

    121023

  • UT code for WoS article

    000537880200025

  • EID of the result in the Scopus database

    2-s2.0-85083031224