Probing plant membranes with FM dyes: tracking, dragging or blocking?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F10%3A10000449" target="_blank" >RIV/00216208:11310/10:10000449 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/10:00343962

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Probing plant membranes with FM dyes: tracking, dragging or blocking?

Original language description

The goal of this manuscript is to describe unexplored effects of the FM styryl dyes, which are widely used probes that label processes of vesicle trafficking in eukaryotic cells. Although there are few reports on the effect of styryl dyes on membrane fluidity and the activity of mammalian receptors, FM dyes have been considered as reliable tools for tracking of plant endocytosis. Using plasma membrane-localized transporters for the plant hormone auxin in tobacco BY-2 and Arabidopsis thaliana cell suspensions, we show that routinely used concentrations of FM 4-64 and FM 5-95 trigger transient re-localization of these proteins, and FM 1-43 affects their activity. The active process of re-localization is blocked neither by inhibitors of endocytosis nor bycytoskeletal drugs. It does not occur in A. thaliana roots and depends on the degree of hydrophobicity of a particular FM dye. Our results emphasize the need for circumspection during in vivo studies of membrane proteins performed using

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Journal

ISSN

0960-7412

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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