Using FM dyes to study endomembranes and their dynamics in plants and cell suspensions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00509656" target="_blank" >RIV/61389030:_____/19:00509656 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-9469-4_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-9469-4_11</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-9469-4_11" target="_blank" >10.1007/978-1-4939-9469-4_11</a>

Result language
angličtina
Original language name
Using FM dyes to study endomembranes and their dynamics in plants and cell suspensions
Original language description
FM (Fei-Mao) styryl dyes are commonly used for the fluorescence imaging of plasma membrane (PM) and endocytosis in vivo. Thanks to their amphiphilic character, these dyes are incorporated in the outer leaflet of the PM lipid bilayer and emit fluorescence in its hydrophobic environment. The endocytic pathway of FM dye uptake starts with rapid PM staining and continues in PM invaginations and membrane vesicles during endocytosis, followed by staining of trans-Golgi network (TGN) and ending in tonoplast (vacuolar membrane). FM dyes do not stain endoplasmic reticulum and nuclear membrane. The time-lapse fluorescence microscopy could track endocytic vesicles and characterize the rate of endocytosis in vivo. On the other hand, fixable FM dyes (FX) can be used for the visualization of particular steps in the FM dye uptake in situ. Staining with FM dyes and subsequent microscopic observations could be performed on both tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and destaining in root tip of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION
ISBN
978-1-4939-9469-4
Number of pages of the result
15
Pages from-to
"Roč. 1992 (2019)"
Number of pages of the book
380
Publisher name
HUMANA PRESS INC.
Place of publication
TOTOWA
UT code for WoS chapter
000487932800012

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