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Using FM dyes to study endomembranes and their dynamics in plants and cell suspensions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F19%3A00509656" target="_blank" >RIV/61389030:_____/19:00509656 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/978-1-4939-9469-4_11" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-9469-4_11</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-4939-9469-4_11" target="_blank" >10.1007/978-1-4939-9469-4_11</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Using FM dyes to study endomembranes and their dynamics in plants and cell suspensions

  • Original language description

    FM (Fei-Mao) styryl dyes are commonly used for the fluorescence imaging of plasma membrane (PM) and endocytosis in vivo. Thanks to their amphiphilic character, these dyes are incorporated in the outer leaflet of the PM lipid bilayer and emit fluorescence in its hydrophobic environment. The endocytic pathway of FM dye uptake starts with rapid PM staining and continues in PM invaginations and membrane vesicles during endocytosis, followed by staining of trans-Golgi network (TGN) and ending in tonoplast (vacuolar membrane). FM dyes do not stain endoplasmic reticulum and nuclear membrane. The time-lapse fluorescence microscopy could track endocytic vesicles and characterize the rate of endocytosis in vivo. On the other hand, fixable FM dyes (FX) can be used for the visualization of particular steps in the FM dye uptake in situ. Staining with FM dyes and subsequent microscopic observations could be performed on both tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and destaining in root tip of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10601 - Cell biology

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2019

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION

  • ISBN

    978-1-4939-9469-4

  • Number of pages of the result

    15

  • Pages from-to

    "Roč. 1992 (2019)"

  • Number of pages of the book

    380

  • Publisher name

    HUMANA PRESS INC.

  • Place of publication

    TOTOWA

  • UT code for WoS chapter

    000487932800012