The use of FM dyes to analyze plant endocytosis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F14%3A00433745" target="_blank" >RIV/61389030:_____/14:00433745 - isvavai.cz</a>
Result on the web
<a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271" target="_blank" >http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=25117271</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-1420-3_1" target="_blank" >10.1007/978-1-4939-1420-3_1</a>
Alternative languages
Result language
angličtina
Original language name
The use of FM dyes to analyze plant endocytosis
Original language description
FM (Fei-Mao) styryl dyes are compounds of amphiphilic character that are used for the fluorescence tracking of endocytosis and related processes, i.e., the internalization of membrane vesicles from the plasma membrane (PM) and dynamics of endomembranes.Staining with FM dyes and subsequent microscopical observations could be performed both on the tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and de-staining in root epidermal cells of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells. The progression of FM dye uptake, reflected by an increased amount of the dye in the endosomal compartments, is monitored under the fluorescence microscope in a time-lapse manner. The data obtained can be used for the characterization of the rate of endocytosis and the function of components of endosomal recycling machinery.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Plant Endosomes - Methods and Protocols
ISBN
978-1-4939-1419-7
Number of pages of the result
11
Pages from-to
1-11
Number of pages of the book
283
Publisher name
Humana Press
Place of publication
Totowa
UT code for WoS chapter
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