Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10099223" target="_blank" >RIV/00216208:11310/11:10099223 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.molbiopara.2010.12.011" target="_blank" >http://dx.doi.org/10.1016/j.molbiopara.2010.12.011</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molbiopara.2010.12.011" target="_blank" >10.1016/j.molbiopara.2010.12.011</a>
Alternative languages
Result language
angličtina
Original language name
Fluorescence in situ hybridization (FISH) mapping of single copy genes on Trichomonas vaginalis chromosomes
Original language description
The highly repetitive nature of the Trichomonas vaginalis genome and massive expansion of various gene families has caused difficulties in genome assembly and has hampered genome mapping. Here, we adapted fluorescence in situ hybridization (FISH) for T.vaginalis, which is sensitive enough to detect single copy genes on metaphase chromosomes. Sensitivity of conventional FISH, which did not allow single copy gene detection in T. vaginalis, was increased by means of tyramide signal amplification. Two selected single copy genes, coding for serine palmitoyltransferase and tryptophanase, were mapped to chromosome land II, respectively, and thus could be used as chromosome markers. This established protocol provides an amenable tool for the physical mappingof the T. vaginalis genome and other essential applications, such as development of genetic markers for T. vaginalis genotyping.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LC07032" target="_blank" >LC07032: Center for Functional Genetics</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Biochemical Parasitology
ISSN
0166-6851
e-ISSN
—
Volume of the periodical
176
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
3
Pages from-to
135-137
UT code for WoS article
000288629800011
EID of the result in the Scopus database
—