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Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00365945" target="_blank" >RIV/61389030:_____/11:00365945 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s10577-011-9235-2" target="_blank" >http://dx.doi.org/10.1007/s10577-011-9235-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10577-011-9235-2" target="_blank" >10.1007/s10577-011-9235-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes

  • Original language description

    Various genetic linkage maps have been established and markers linked to resistance genes been identified in cultivated chickpea. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LC06004" target="_blank" >LC06004: Integration of research activities to study the plant genome</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Chromosome Research

  • ISSN

    0967-3849

  • e-ISSN

  • Volume of the periodical

    19

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    729-739

  • UT code for WoS article

    000300089200003

  • EID of the result in the Scopus database