Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00365945" target="_blank" >RIV/61389030:_____/11:00365945 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10577-011-9235-2" target="_blank" >http://dx.doi.org/10.1007/s10577-011-9235-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10577-011-9235-2" target="_blank" >10.1007/s10577-011-9235-2</a>
Alternative languages
Result language
angličtina
Original language name
Integration of genetic and physical maps of the chickpea (Cicer arietinum L.) genome using flow-sorted chromosomes
Original language description
Various genetic linkage maps have been established and markers linked to resistance genes been identified in cultivated chickpea. However, until now, only one linkage group (LG) has been assigned to a specific chromosome. In the present work, mitotic chromosomes were sorted using flow cytometry and used as template for PCR with primers designed for genomic regions flanking microsatellites. These primers amplify sequence-tagged microsatellite site markers. To establish a set of preferably diagnostic cytogenetic markers, the genomic distribution of various probes was verified using FISH. Moreover, a partial genomic bacterial artificial chromosome (BAC) library was constructed and putative single/low-copy BAC clones weremapped cytogenetically. As a result, two clones were identified localizing specifically to chromosomes E and H, for which no cytogenetic markers were yet available.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LC06004" target="_blank" >LC06004: Integration of research activities to study the plant genome</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromosome Research
ISSN
0967-3849
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
729-739
UT code for WoS article
000300089200003
EID of the result in the Scopus database
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