Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10100481" target="_blank" >RIV/00216208:11310/11:10100481 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/11:00364976 RIV/68378050:_____/11:00364976 RIV/61388963:_____/11:00364976

Result on the web

<a href="http://dx.doi.org/10.1021/bm200897b" target="_blank" >http://dx.doi.org/10.1021/bm200897b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/bm200897b" target="_blank" >10.1021/bm200897b</a>

Result language

angličtina

Original language name

Coiled coil peptides as universal linkers for the attachment of recombinant proteins to polymer therapeutics

Original language description

We have designed, synthesized, and characterized peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as conWe prepared peptides containing four repeats of the sequences VAALEKE (peptide E) or VAALKEK (peptide K). While the peptides alone adopt in aqueous solutions a random coil conformation, their equimolar mixture forms heterodimeric coiled coils as confirmed by CD spectroscopy. A terminal azide group enabled conjugation of the peptide with a synthetic drug carrier based on the N-(2-hydroxypropyl)methacrylamide copolymer containing propargyl groups using "click" chemistry. When incorporated into the polymer drug carrier, peptide E formed a stable noncovalent complex with peptide K belonging to a recombinant single-chain fragment (scFv) of the M75 antibody. The complex mediated a noncovalent linkage between th

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CC - Organic chemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomacromolecules

ISSN

1525-7797

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

3645-3655

UT code for WoS article

000295602600032

EID of the result in the Scopus database

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