Phototoxic effect of TPPS(4) and MgTPPS(4) on DNA fragmentation of HeLa cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10105069" target="_blank" >RIV/00216208:11310/11:10105069 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/11:33118366
Result on the web
<a href="http://dx.doi.org/10.1016/j.tiv.2010.11.005" target="_blank" >http://dx.doi.org/10.1016/j.tiv.2010.11.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.tiv.2010.11.005" target="_blank" >10.1016/j.tiv.2010.11.005</a>

Result language
angličtina
Original language name
Phototoxic effect of TPPS(4) and MgTPPS(4) on DNA fragmentation of HeLa cells
Original language description
Photodynamic therapy (PDT) is an alternative method of tumour treatment. It is based on a photochemical reaction of a photosensitizer, irradiation, and O(2) which converts to cytotoxic (1)O(2) and other forms of reactive oxygen species (ROS). The comet assay (also called single-cell gel electrophoresis. SCGE) is a sensitive, simple and quantitative technique for detection of DNA damage. In our study we investigated the phototoxicity of the two porphyrin photosensitizers. TPPS4 and MgTPPS4, on HeLa cells. Three different radiation doses and six different concentrations of the photosensitizers were used. Our results show that the DNA of the cells treated with the TPPS4 and MgTPPS4 at the concentrations higher than 51 mu M was highly fragmented indicatinga strong phototoxic effect resulting in a cell apoptosis. On the base of our results we can hypothesize that even the irradiation dose of 1 J cm(-2) is sufficient enough to provoke the DNA fragmentation.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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