Ecotin-like serine peptidase inhibitor ISP1 of Leishmania major plays a role in flagellar pocket dynamics and promastigote differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10123309" target="_blank" >RIV/00216208:11310/12:10123309 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1462-5822.2012.01798.x" target="_blank" >http://dx.doi.org/10.1111/j.1462-5822.2012.01798.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1462-5822.2012.01798.x" target="_blank" >10.1111/j.1462-5822.2012.01798.x</a>

Result language

angličtina

Original language name

Ecotin-like serine peptidase inhibitor ISP1 of Leishmania major plays a role in flagellar pocket dynamics and promastigote differentiation

Original language description

Leishmania ISPs are ecotin-like natural peptide inhibitors of trypsin-family serine peptidases, enzymes that are absent from the Leishmania genome. This led to the proposal that ISPs inhibit host serine peptidases and we have recently shown that ISP2 inhibits neutrophil elastase, thereby enhancing parasite survival in murine macrophages. In this study we show that ISP1 has less serine peptidase inhibitory activity than ISP2, and in promastigotes both are generally located in the cytosol and along the flagellum. However, in haptomonad promastigotes there is a prominent accumulation of ISP1 and ISP2 in the hemidesmosome and for ISP2 on the cell surface. An L. major mutant deficient in all three ISP genes (?isp1/2/3) was generated and compared with ?isp2/3 mutants to elucidate the physiological role of ISP1. In in vitro cultures, the ?isp1/2/3 mutant contained more haptomonad, nectomonad and leptomonad promastigotes with elongated flagella and reduced motility compared with ?isp2/3 popula

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular Microbiology

ISSN

1462-5814

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

1271-1286

UT code for WoS article

000306405000011

EID of the result in the Scopus database

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