Stability of influenza virus as evaluated by integrity of its RNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10125432" target="_blank" >RIV/00216208:11310/12:10125432 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.4149/av_2012_02_125" target="_blank" >http://dx.doi.org/10.4149/av_2012_02_125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/av_2012_02_125" target="_blank" >10.4149/av_2012_02_125</a>

Result language

angličtina

Original language name

Stability of influenza virus as evaluated by integrity of its RNA

Original language description

Various methods of handling samples of avian influenza prior to detecting influenza viruses can significantly influence both, the detection of the virus and the quantification of viral nucleic acids. The quantity of influenza viral RNA remaining in different collecting buffers and kept at temperatures of -20 degrees C, +4 degrees C or +22 degrees C for various lengths of time, was determined. The quantity of viral RNA remained the same for 120 days at -20 degrees C, but decreased when the samples were stored at either +4 degrees C or +22 degrees C. The quantity of RNA was influenced by the composition of the collecting buffer. The influenza virus sample that is to be used for RNA quantification can be stored at +4 degrees C and freeze and thaw cycles should be avoided during transport. Our results clearly indicate that the quality and quantity of influenza virus nucleic acid depends on the chemical composition of used buffer and also that the samples can be protected from degradation e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/SP%2F2D3%2F60%2F08" target="_blank" >SP/2D3/60/08: Integrated research of the effects and consequences of artificially reared mallards (Anas platyrhynchos) on wetland ecosystems with the application of advanced ecological and molecular techniques.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Virologica

ISSN

0001-723X

e-ISSN

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Volume of the periodical

56

Issue of the periodical within the volume

2

Country of publishing house

SK - SLOVAKIA

Number of pages

4

Pages from-to

125-128

UT code for WoS article

000307860400005

EID of the result in the Scopus database

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