Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10130256" target="_blank" >RIV/00216208:11310/12:10130256 - isvavai.cz</a>

Alternative codes found

RIV/67985939:_____/12:00385584 RIV/60076658:12310/12:43883647

Result on the web

<a href="http://dx.doi.org/10.1007/s10577-012-9277-0" target="_blank" >http://dx.doi.org/10.1007/s10577-012-9277-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10577-012-9277-0" target="_blank" >10.1007/s10577-012-9277-0</a>

Result language

angličtina

Original language name

Glycerol-treated nuclear suspensions-an efficient preservation method for flow cytometric analysis of plant samples

Original language description

Flow cytometry (FCM) has been widely used in plant science to determine the amount of nuclear DNA, either in absolute units or in relative terms, as an indicator of ploidy. The requirement for fresh material in some applications, however, limits the value of FCM in field research, including plant biosystematics, ecology and population biology. Dried plant samples have proven to be a suitable alternative in some cases (large-scale ploidy screening) although tissue dehydration is often associated with a decrease in the quality of FCM analysis. The present study tested, using time-scale laboratory and in situ field experiments, the applicability of glycerol-treated nuclear suspension for DNA flow cytometry. We demonstrate that plant nuclei preserved in ice-cold buffer + glycerol solution remain intact for at least a few weeks and provide estimates of nuclear DNA content that are highly comparable and of similar quality to those obtained from fresh tissue. The protocol is compatible with b

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chromosome Research

ISSN

0967-3849

e-ISSN

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Volume of the periodical

20

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

303-315

UT code for WoS article

000302325300005

EID of the result in the Scopus database

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