Plant material selection, collection, preservation, and storage for nuclear DNA content estimation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00566766" target="_blank" >RIV/67985939:_____/22:00566766 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/22:10456548 RIV/60076658:12310/22:43905646
Result on the web
<a href="https://doi.org/10.1002/cyto.a.24482" target="_blank" >https://doi.org/10.1002/cyto.a.24482</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.24482" target="_blank" >10.1002/cyto.a.24482</a>
Alternative languages
Result language
angličtina
Original language name
Plant material selection, collection, preservation, and storage for nuclear DNA content estimation
Original language description
In theory, any plant tissue providing intact nuclei in sufficient quantity is suitable for nuclear DNA content estimation using flow cytometry (FCM). While this certainly opens a wide variety of possible applications of FCM, especially when compared to classical karyological techniques restricted to tissues with active cell division, tissue selection and quality may directly affect the precision (and sometimes even reliability) of FCM measurements. It is usually convenient to first consider the goals of the study to either aim for the highest possible accuracy of estimates (e.g., for inferring genome size, detecting homoploid intraspecific genome size variation, aneuploidy, among others), or to decide that histograms of reasonable resolution provide sufficient information (e.g., ploidy level screening within a single model species). Here, a set of best practices guidelines for selecting the optimal plant tissue for FCM analysis, sampling of material, and material preservation and storage are provided. In addition, factors potentially compromising the quality of FCM estimates of nuclear DNA content and data interpretation are discussed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/GA20-22783S" target="_blank" >GA20-22783S: Genome duplication as an imperfect barrier in speciation? Evolutionary drivers and consequences of inter-ploidy introgression in natural populations</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry. Part A
ISSN
1552-4922
e-ISSN
1552-4930
Volume of the periodical
101
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
737-748
UT code for WoS article
000672491100001
EID of the result in the Scopus database
2-s2.0-85109739806