Application-based guidelines for best practices in plant flow cytometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985939%3A_____%2F22%3A00561312" target="_blank" >RIV/67985939:_____/22:00561312 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/22:00125097 RIV/60076658:12310/22:43904742 RIV/00216208:11310/22:10474746
Result on the web
<a href="https://doi.org/10.1002/cyto.a.24499" target="_blank" >https://doi.org/10.1002/cyto.a.24499</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.24499" target="_blank" >10.1002/cyto.a.24499</a>

Result language
angličtina
Original language name
Application-based guidelines for best practices in plant flow cytometry
Original language description
Flow cytometry (FCM) is currently the most widely-used method to establish nuclear DNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are sufficient for most plant applications, are commercially available at a reasonable price, the number of laboratories equipped with these instruments, and consequently new FCM users, has greatly increased over the last decade. This paper meets an urgent need for comprehensive recommendations for best practices in FCM for different plant science applications. We discuss advantages and limitations of establishing plant ploidy, genome size, DNA base composition, cell cycle activity, and level of endoreduplication. Applications of such measurements in plant systematics, ecology, molecular biology research, reproduction biology, tissue cultures, plant breeding, and seed sciences are described. Advice is included on how to obtain accurate and reliable results, as well as how to manage troubleshooting that may occur during sample preparation, cytometric measurements, and data handling. Each section is followed by best practice recommendations, tips as to what specific information should be provided in FCM papers are also provided.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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