Best practices for instrument settings and raw data analysis in plant flow cytometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43907426" target="_blank" >RIV/60076658:12310/23:43907426 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.24798" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.24798</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.24798" target="_blank" >10.1002/cyto.a.24798</a>
Alternative languages
Result language
angličtina
Original language name
Best practices for instrument settings and raw data analysis in plant flow cytometry
Original language description
Flow cytometry (FCM) is now the most widely used method to determine ploidy levels and genome size of plants. To get reliable estimates and allow reproducibility of measurements, the methodology should be standardized and follow the best practices in the field. In this article, we discuss instrument calibration and quality control and various instrument and acquisition settings (parameters, flow rate, number of events, scales, use of discriminators, peak positions). These settings must be decided before measurements because they determine the amount and quality of the data and thus influence all downstream analyses. We describe the two main approaches to raw data analysis (gating and histogram modeling), and we discuss their advantages and disadvantages. Finally, we provide a summary of best practice recommendations for data acquisition and raw data analysis in plant FCM.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cytometry Part A
ISSN
1552-4922
e-ISSN
1552-4930
Volume of the periodical
103
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
953-966
UT code for WoS article
001079755300001
EID of the result in the Scopus database
2-s2.0-85173934506