PiB-Conjugated, Metal-Based Imaging Probes: Multimodal Approaches for the Visualization of beta-Amyloid Plaques

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10139591" target="_blank" >RIV/00216208:11310/13:10139591 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/ml400042w" target="_blank" >http://dx.doi.org/10.1021/ml400042w</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ml400042w" target="_blank" >10.1021/ml400042w</a>

Result language
angličtina
Original language name
PiB-Conjugated, Metal-Based Imaging Probes: Multimodal Approaches for the Visualization of beta-Amyloid Plaques
Original language description
In an effort toward the visualization of beta-amyloid plaques by in vivo imaging techniques, we have conjugated an optimized derivative of the Pittsburgh compound B (PiB), a well-established marker of Afi plaques, to DO3A-monoamide that is capable of forming stable, noncharged complexes with different trivalent metal ions including Gd3+ for MRI and In-111(3+). for SPECT applications. Proton relaxivity measurements evidenced binding of Gd(DO3A-PiB) to the amyloid peptide A beta(1-40) and to human serum albumin, resulting in a two- and four-fold relaidvity increase, respectively. Ex vivo immunohistochemical studies showed that the DO3A-PiB complexes selectively target A beta plaques on Alzheimer's disease human brain tissue. Ex vivo biodistribution dataobtained for the In-111-analogue pointed to a moderate blood-brain barrier (BBB) penetration in adult male Swiss mice (without amyloid deposits) with 0.36% ID/g in the cortex at 2 min postinjection.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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