Functional expression and subcellular localization of pea polymorphic isoflavone synthase CYP93C18

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190749" target="_blank" >RIV/00216208:11310/13:10190749 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/13:00420942 RIV/60461373:22330/13:43895421

Result on the web

<a href="http://dx.doi.org/10.1007/s10535-013-0344-y" target="_blank" >http://dx.doi.org/10.1007/s10535-013-0344-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10535-013-0344-y" target="_blank" >10.1007/s10535-013-0344-y</a>

Result language

angličtina

Original language name

Functional expression and subcellular localization of pea polymorphic isoflavone synthase CYP93C18

Original language description

Isoflavone synthase (IFS; CYP93C) plays a key role in the biosynthesis of phenolic secondary metabolites, isoflavonoids. These compounds, which are well-known for their benefits to human health and plant defence, are produced mostly in legumes. However,more than 200 of them have been described in 59 other plant families without any knowledge of their respective IFS orthologue genes (with the sole exception of sugar beet). In this study, we selected IFS from Pisum sativum L. (CYP93C18) for functional expression. CYP93C18 was isolated, cloned, and introduced into Arabidopsis thaliana. The presence of the gene was shown by Southern blot analysis and its expression in the transgenic Arabidopsis was proven by RT-PCR and Western blots. The functional activity of the heterologous IFS was verified by HPLC-MS analysis of the metabolite levels: the isoflavone genistein and its derivatives tectorigenin and biochanin A were detected in the overexpressing lines. In addition, 35S::CYP93C18::GFP fus

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

635-645

UT code for WoS article

000324878300006

EID of the result in the Scopus database

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