Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191562" target="_blank" >RIV/00216208:11310/13:10191562 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/13:00422357
Result on the web
<a href="http://dx.doi.org/10.1007/s11099-013-0051-0" target="_blank" >http://dx.doi.org/10.1007/s11099-013-0051-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11099-013-0051-0" target="_blank" >10.1007/s11099-013-0051-0</a>
Alternative languages
Result language
angličtina
Original language name
Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis
Original language description
Photosystem (PS) II particles retaining a high rate of O-2 evolution were isolated from the mesophilic filamentous cyanobacterium, Spirulina platensis. To achieve high production of PSII complexes in the cells, irradiance from halogen incandescent lampswas used. Disruption of cells by vibration of glass beads proved to be the most suitable procedure for isolation of thylakoid membranes. The selectivity of detergents for PSII particle preparation rose in the order of Triton X-100 < decyl-beta-D-glucopyranoside < dodecyldimethyl-aminooxide < n-heptyl-beta-D-thioglucoside < N-dodecyl-N,N-dimethylammonio-3-propane sulphonate < n-octyl-beta-thioglycoside < octylglucoside < n-dodecyl-beta-D-maltoside. The last four detergents yielded extracts, from which pure PSII particles not contaminated by PSI complexes could be obtained by sucrose-gradient centrifugation (20-45%) at the 43% sucrose level.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photosynthetica
ISSN
0300-3604
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
4
Country of publishing house
DE - GERMANY
Number of pages
24
Pages from-to
517-530
UT code for WoS article
000327507500006
EID of the result in the Scopus database
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