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Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191562" target="_blank" >RIV/00216208:11310/13:10191562 - isvavai.cz</a>

  • Alternative codes found

    RIV/61388971:_____/13:00422357

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s11099-013-0051-0" target="_blank" >http://dx.doi.org/10.1007/s11099-013-0051-0</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11099-013-0051-0" target="_blank" >10.1007/s11099-013-0051-0</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Isolation and characterization of oxygen-evolving photosystem II particles and photosystem II core complex from the filamentous cyanobacterium Spirulina platensis

  • Original language description

    Photosystem (PS) II particles retaining a high rate of O-2 evolution were isolated from the mesophilic filamentous cyanobacterium, Spirulina platensis. To achieve high production of PSII complexes in the cells, irradiance from halogen incandescent lampswas used. Disruption of cells by vibration of glass beads proved to be the most suitable procedure for isolation of thylakoid membranes. The selectivity of detergents for PSII particle preparation rose in the order of Triton X-100 < decyl-beta-D-glucopyranoside < dodecyldimethyl-aminooxide < n-heptyl-beta-D-thioglucoside < N-dodecyl-N,N-dimethylammonio-3-propane sulphonate < n-octyl-beta-thioglycoside < octylglucoside < n-dodecyl-beta-D-maltoside. The last four detergents yielded extracts, from which pure PSII particles not contaminated by PSI complexes could be obtained by sucrose-gradient centrifugation (20-45%) at the 43% sucrose level.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Photosynthetica

  • ISSN

    0300-3604

  • e-ISSN

  • Volume of the periodical

    51

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    24

  • Pages from-to

    517-530

  • UT code for WoS article

    000327507500006

  • EID of the result in the Scopus database