A Computational Study of the Glycine-Rich Loop of Mitochondrial Processing Peptidase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191950" target="_blank" >RIV/00216208:11310/13:10191950 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/13:00423430 RIV/61989592:15310/13:33148395

Result on the web

<a href="http://dx.doi.org/10.1371/journal.pone.0074518" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0074518</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0074518" target="_blank" >10.1371/journal.pone.0074518</a>

Result language

angličtina

Original language name

A Computational Study of the Glycine-Rich Loop of Mitochondrial Processing Peptidase

Original language description

An all atomic, non-restrained molecular dynamics (MD) simulation in explicit water was used to study in detail the structural features of the highly conserved glycine-rich loop (GRL) of the a-subunit of the yeast mitochondrial processing peptidase (MPP)and its importance for the tertiary and quaternary conformation of MPP. Wild-type and GRL-deleted MPP structures were studied using non-restrained MD simulations, both in the presence and the absence of a substrate in the peptidase active site. TargetedMD simulations were employed to study the mechanism of substrate translocation from the GRL to the active site. We demonstrate that the natural conformational flexibility of the GRL is crucial for the substrate translocation process from outside the enzyme towards the MPP active site. We show that the a-helical conformation of the substrate is important not only during its initial interaction with MPP (i.e. substrate recognition), but also later, at least during the first third of the su

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS ONE

ISSN

1932-6203

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

9

Country of publishing house

ES - SPAIN

Number of pages

13

Pages from-to

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UT code for WoS article

000324408400082

EID of the result in the Scopus database

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