Glycine-rich loop of mitochondrial processing peptidase ?-subunit is responsible for substrate recognition by a mechanism analogous to mitochondrial receptor Tom20

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F10%3A00340832" target="_blank" >RIV/61388971:_____/10:00340832 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Glycine-rich loop of mitochondrial processing peptidase ?-subunit is responsible for substrate recognition by a mechanism analogous to mitochondrial receptor Tom20

Original language description

Tryptophan fluorescence measurements were used to characterize the local dynamics of the highly conserved glycine-rich loop (GRL) of the mitochondrial processing peptidase (MPP) subunit in the presence of the substrate precursor. Reporter tryptophan residue was introduced into the GRL of the yeast MPP (Y299W) or at a proximal site (Y303W). Timeresolved and steady-state fluorescence spectroscopy demonstrated that for Trp299, the primary contact with the yeast malate dehydrogenase precursor evokes a change of the local GRL mobility. Moreover, time-resolved measurements showed that a functionless MPP with a single-residue deletion in the loop (Y303W/G292) is defective particularly in the primary contact with substrate

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/IAA501110631" target="_blank" >IAA501110631: Evolution and biogenesis of mitochondria-related organelles: hydrogenosomes and mitosomes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Molecular Biology

ISSN

0022-2836

e-ISSN

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Volume of the periodical

396

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

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UT code for WoS article

000275691500002

EID of the result in the Scopus database

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